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#1. GCaMP
It is a synthetic fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light-chain kinase. When bound to Ca, ...
#2. Crystal Structures of the GCaMP Calcium Sensor Reveal ...
The GCaMP sensor is composed of a circularly permuted enhanced GFP (cpEGFP) moiety attached to the calcium-binding protein calmodulin (CaM) and ...
#3. GCaMP - an overview | ScienceDirect Topics
For example, GCaMP is an engineered GECI that reports changes in calcium based on a fusion between green fluorescent protein (GFP) and the calcium binding ...
#4. Deciphering the molecular mechanism responsible for ...
The mechanism of GCaMP6m is more complicated than a simple "dim" to "bright" transition of the chromophore. GCaMP fluorescence is typically imaged with ~480 nm ...
#5. GCaMP application in neuroscience - YouTube
This video will talk about a genetically encoded calcium sensor GCaMP. GCaMP is a genetically encoded calcium indicator (GECI) initially ...
#6. Fast and sensitive GCaMP calcium indicators for imaging ...
Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design. J. Biol. Chem ...
#7. Genetically encoded calcium indicators (GECIs)
Akerboom J., et. al. Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design. J. Biol. Chem.
#8. Fast and sensitive GCaMP calcium indicators for neuronal ...
GCaMP, the prototypical single-fluorophore GECI, consists of a circularly permuted green fluorescent protein (cpGFP) flanked by the Ca2+-binding ...
#9. Visualization and analysis of neural activity|IINO Laboratory
Figure 1: Mechanism of GCaMP. GCaMP is composed of improved GFP, Calmodulin (CaM, calcium-binding protein) and M13 peptide. GCaMP shows fluorescence only when ...
#10. Chronic Ca2+ imaging of cortical neurons with long-term ...
... GCaMP-X, serving as the major candidate mechanism of cellular GCaMP toxicities. Acute sensory responses monitored by viral expression of ...
#11. Mechanism of kisspeptin neuron synchronization for ...
An acute brain slice preparation maintaining synchronized ARNKISS neuron burst firing was used alongside in vivo GCaMP GRIN lens microendoscope and fiber ...
#12. Crystal Structures of the GCaMP Calcium Sensor Reveal ...
Crystal Structures of the GCaMP Calcium Sensor Reveal the Mechanism of Fluorescence Signal Change and Aid Rational Design. Full Record; Other ...
#13. Improved calcium sensor GCaMP-X overcomes the ...
In summary, CaM/CaV1 gating and signaling mechanisms are elucidated for GCaMP side- effects, while allowing the development of GCaMP-X to appropriately monitor ...
#14. The kinetic mechanisms of fast-decay red-fluorescent ...
The mechanistic differences explain the generally slower off-kinetics of jRCaMP1a- type indicators compared with GCaMP-s and jRGECO1a-type. GECI: the ...
#15. Genetic visualization with an improved GCaMP calcium ...
These findings may suggest the existence of a common mechanism for motor circuits in the vertebrate developing spinal cord. GCaMP Technology and ...
#16. Optimization of a GCaMP Calcium Indicator for Neural ...
... mechanism. The primary utility of GCaMP crystal structures has been to delineate the protein domain proto-interfaces and the GFP chromophore ...
#17. Investigation of Pain Mechanisms by Calcium Imaging ...
Recently, an optical imaging technique has been developed using the GECIs GCaMP3 and GCaMP6s genetically expressed in primary sensory neurons ...
#18. Fast and sensitive GCaMP calcium indicators for imaging ...
Generally, attempts to improve ΔF/F0 are associated with a slowing of kinetics. The mechanisms underlying this trade-off are not simply due to ...
#19. Unraveling Synaptic GCaMP Signals: Differential ...
GCaMP is an optogenetic Ca 2+ sensor widely used for monitoring neuronal activities but the precise physiological implications of GCaMP signals ...
#20. Genetically Encoded Calcium Indicators Can Impair ...
Mechanisms regulating dendritic arbor patterning. ... Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the ...
#21. Quantitative comparison of novel GCaMP-type genetically ...
Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design. J. Biol. Chem ...
#22. Calcium imaging for analgesic drug discovery
6, GCaMP2, GCaMP3, GCaMP-HS, Fast- GCaMPs, GCaMP5, and ... GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design.
#23. (PDF) imaging Neural Activity in Worms, Flies and Mice ...
Although their expression is restricted to the cytosol, reports suggest that GCaMPs can translocate to nuclei in certain neurons, but the mechanism or timing is ...
#24. Search by PDB author
X-RAY DIFFRACTION (2.8 Å). Cite: Crystal Structures of the GCaMP Calcium Sensor Reveal the Mechanism of Fluorescence Signal Change and Aid Rational Design J ...
#25. Engineering-Fluorescent-Calcium-Sensor-Proteins-for- ...
of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design. J Biol Chem 284:6455–6464. Akerboom J, Chen TW ...
#26. Design and mechanistic insight into ultrafast calcium ...
GCaMP6s and GCaMP6f generation of GCaMP-type calcium indicators. We hypothesized that GCaMP-s have a common kinetic mechanism with a rate-limiting process ...
#27. Crystal Structures of the GCaMP Calcium Sensor Reveal ...
Crystal Structures of the GCaMP Calcium Sensor Reveal the Mechanism of Fluorescence Signal Change and Aid Rational Design*S⃞. @article ...
#28. Crystal structures of the GCaMP calcium sensor reveal ...
This series of structures provides insight into the mechanism of Ca2+-induced fluorescence change. Upon calcium binding, the calmodulin (CaM) ...
#29. Imaging neural activity in worms, flies and mice with ...
Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design. J. Biol. Chem.284, 6455–6464 ...
#30. Monitoring astrocyte calcium microdomains with improved ...
However, we emphasise that the GCaMP moiety in Lck-GCaMP3 ... (2009) Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal ...
#31. gcamp calcium indicators: Topics by ...
Studying the mechanism of neurostimulation by infrared laser light using GCaMP6s and Rhodamine B imaging · NASA Astrophysics Data System (ADS). Moreau, David ...
#32. Investigation of Pain Mechanisms by Calcium Imaging ...
Researchers can now induce GCaMP expression in the mouse genome using viral or gene knock-in approaches and observe the activity of populations of cells in the ...
#33. Publications
Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design. J. Biol. Chem., Mar 2009; 284 ...
#34. Structural insight into enhanced calcium indicator GCaMP3 ...
ER. Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design . J Biol Chem. 2009. 284. ,. 6455.
#35. Imaging and analysis of genetically encoded calcium ...
Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design. J Biol Chem. 2009 ...
#36. Multiscale photoacoustic tomography of neural activities ...
In general, GCaMP consists of a circularly permuted EGFP, an M13 fragment of myosin light chain kinase, and a calcium binding site of calmodulin. Action ...
#37. Genetically encoded calcium indicators and methods of use
Example 9 GCaMP Variants and Methods of Screening GCaMP Variants; [0081] ... Server with mechanism for reducing internal resources associated with a selected ...
#38. Research Achievements-School of Medicine ...
... GCaMP' in Nature Communications.GCaMP, one p... Zeng's group revealed intrinsic immune response to traumatic injuries in neuron...2018-04-17. Dr. Wenwen Zeng ...
#39. Improved calcium sensor GCaMP-X overcomes the ...
In summary, CaM/Ca<jats:sub>V</jats:sub>1 gating and signaling mechanisms are elucidated for GCaMP side-effects, while allowing the development of GCaMP-X to ...
#40. Viral Vectors 101: Calcium Sensors
There are three GCaMP types based on their kinetics: GCaMPf (fast) ... Structure-and mechanism-guided design of single fluorescent protein-based ...
#41. Optimization of a GCaMP Calcium Indicator ... - Harvard DASH
GCaMP3 across all stimuli frequencies (n 6, 10 NMJs/GCaMP, ... of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational ...
#42. Genetically Encoded Bright Ca2+ Probe Applicable for ...
Crystal Structures of the GCaMP Calcium Sensor Reveal the Mechanism of Fluorescence Signal Change and Aid Rational Design. Journal of ...
#43. Recombinant Construct: P{LexAop-GCaMP-IRES-GCaMP}
P{LexAop-GCaMP-IRES-GCaMP}. FlyBase ID. FBtp0053414. Feature type ... A presynaptic gain control mechanism fine-tunes olfactory behavior.[FBrf0205669].
#44. The Functional Characterization of GCaMP3.0 Variants ...
Mitochondria are known to play a key role in controlling [Ca2+]i due to efficient seques- tration and release mechanisms that have been ...
#45. Fiber Photometry Overview
GCaMP fluoresces only when bound to a calcium ion, providing insight into ... The mechanism that causes photodarkening is largely unknown, but several fiber ...
#46. Alex Keyes | Department of Neuroscience and Pharmacology
... mechanism is central sensitization in the spinal cord. I will use neuron- and microglia- specific GCaMP ... GCaMP3 imaging. Society for Neuroscience, Chicago, IL ...
#47. Deciphering the molecular mechanism responsible for ...
GCaMP 6m changes fluorescencepKmechanismGCaMP 6m fluorescenceresponsechromophore protonation stateshiftM 13 peptideGCaMP 6m. Licence.
#48. A Neural Mechanism for Switching between Sleep States
Using a retrograde viral tracing tool (rAAV-retro), they expressed GCaMP into selective DMH neurons based on their projections. To determine ...
#49. An Expanded Palette of Genetically Encoded Ca2+ Indicators
al., Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design. J. Biol. Chem. 284, 6455 (2009).
#50. Photoswitchable Fluorescent Proteins: Mechanisms on ...
The Functional Characterization of GCaMP3.0 Variants Specifically Targeted to Subcellular Domains. Previous Article in Journal. The m6A Methyltransferase ...
#51. 搜索
... GCaMP series. 中文翻译：. 基于单一荧光蛋白的生物传感器的结构和机制指导设计. 基于单个荧光蛋白荧光的变构调制的强度测量遗传编码生物传感器是实现 ...
#52. The kinetic mechanisms of fast-decay red-fluorescent ...
The mechanistic differences explain the generally slower off-kinetics of jRCaMP1a- type indicators compared with GCaMP-s and jRGECO1a-type. GECI: the ...
#53. Separation of hemodynamic signals from GCaMP ...
Coupling mechanism and significance of the BOLD signal: a status report. Annu Rev Neurosci 37: 161–181, 2014. doi:10.1146/annurev-neuro ...
#54. Lin Tian
2012. Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design. J Akerboom, JDV Rivera, MMR ...
#55. Fast and sensitive GCaMP calcium indicators for imaging ...
The mechanisms underlying this trade-off are not simply due to binding affinity for Ca2+ and in general are not well-understood. For example, ...
#56. Genetically Encoded Ca 2+ Indicators in Cardiac Myocytes
Interestingly, the upstroke duration was largely independent of the actual mechanism generating the fluorescence changes. Although GCamP3 ...
#57. Optogenetics: Tools for Controlling and Monitoring Neuronal ...
... GCaMP-type sensing mechanism. Upon calcium binding, conformational changes in the CaM/M13 complex induce fluorescence changes in the circularly permuted GFP ...
#58. Blue Biotechnology: Production and Use of Marine Molecules
... GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design. J. Biol. Chem., 284, 6455–6464. Ahrens, M.B., Orger, M.B. ...
gcamp mechanism 在 GCaMP application in neuroscience - YouTube 的推薦與評價
This video will talk about a genetically encoded calcium sensor GCaMP. GCaMP is a genetically encoded calcium indicator (GECI) initially ... ... <看更多>