關於 barnase gene ，我們在網路上蒐集到這些相關的討論、資訊與評價

#11. Engineered Male Sterility by Early Anther Ablation Using the ...

Genetic engineered male sterility has different applications, ranging from ... This promoter fused to the barnase gene produces full anther ...

#12. (PDF) Development of intron-containing barnase gene ...

PDF | Developing gene constructs with the barnase gene and propagating them in Escherichia coli or Agrobacterium tumefaciens is difficult as ...

#13. Barnase | BCH-GENE-SCBD-14973 | Genetic element

Barnase is a bacterial protein that has ribonuclease activity. It is lethal to the cell when expressed without its inhibitor barstar.

#14. Development of male sterile transgenic lines in rice by ...

2010), the TA29 promoter from Nicotiana tabacum has been successfully used to drive expression of the barnase gene in tapetum tissue. In case of ...

#15. Developing transgenic plants with no seeds by transforming ...

Developing transgenic plants with no seeds by transforming Barnase gene [2012]. Chen Guimin, Chinese Academy of Agricultural Sciences, Wuhan(China), ...

#16. Development of intron-containing barnase gene ... - Springer

Developing gene constructs with the barnase gene and propagating them in Escherichia coli or Agrobacterium tumefaciens is difficult as ...

#17. 1BNR: BARNASE - RCSB PDB

Determination of the three-dimensional solution structure of barnase using nuclear magnetic resonance spectroscopy. ... Gene Names: BARNASE EC: 3.1.27 ...

#18. Part:BBa K1172904 - iGEM Parts

Rnase Ba (Barnase) from Bacillus amyloliquefaciens ... Translation of the barnase gene leads to rapid cell death if the expression of the ...

#19. Barstar (barnase inhibitor) (IPR000468) - InterPro entry

Description. Barstar is a small single chain protein. Barnase is the extracellular ribonuclease. IPR001887. of Bacillus amyloliquefaciens, and barstar its ...

#20. A Phosphate Starvation-Inducible Ribonuclease of Bacillus ...

An amino-acid sequence analysis of the BLi03719-encoding gene showed a high similarity with genes encoding the barnase of Bacillus amyloliquefaciens FZB42 ...

#21. Retransformation of a male sterile barnase line with ... - CORE

the barnase and barstar genes were identified and screened for their male fertility status. ... specific expression of a ribonuclease gene (barnase) and its.

#22. Amino-acid Sequence of Extracellular Ribonuclease (Barnase ...

THE ribonuclease, barnase, produced by Bacillus amyloliquefaciens has ... the stability of various genetic variants and chemically modified, ...

#23. mustard (Brassica juncea L. Czern & Coss) - jstor

transgenics with barnase and barstar genes respectively2"4. ... the combiners by expressing the barnase gene from Ba.

#24. Development of male sterile transgenic lines ... - KoreaScience

The key to development of barnase-barstar transgene based hybrid seed technology is ... cultivar Hayayuki to express the barnase gene in rice transgenic lines.

#25. US8383575B2 - (DI)barnase-barstar complexes

For example the barnase gene of Bacillus amyloliquefaciens codes for an RNase, the barnase, which can be inhibited by a protein, the barstar, ...

#26. A new chimeric toxin composed of pseudomonas exotoxin A ...

Cloning, sequencing and transcription of an inactivated copy of Bacillus amyloliquefaciens extracellular ribonuclease (barnase). Gene. 1986; 40: 231-239.

#27. Methods for Studying the Interaction of Barnase with Its ...

Barnase is a 110-residue extracellular ribonuclease produced by Bacillus amyloliquefaciens. The same organism produces an 89-residue polypeptide, barstar, ...

#28. barnase-barstar gene system | Britannica

Other articles where barnase-barstar gene system is discussed: bacillus: … known ... The gene controlling production of the Bt toxin in B. thuringiensis has ...

#29. a genetic selection for compensating mutations at the barnase ...

Barnase and barstar are trivial names of the extracellular RNase and its ... expression is tightly controlled to keep the mutant barnase gene silent.

#30. Development of barnase/barstar transgenics for hybrid seed ...

... juncea using the barnase-barstar system in conjunction with a selection marker gene ... seeds based on transgenic approach using barnase and barstar genes.

#31. Factors influencing Agrobacterium-mediated embryogenic ...

Factors influencing Agrobacterium-mediated embryogenic callus transformation of Valencia sweet orange (Citrus sinensis) containing the pTA29-barnase gene.

#32. ARCHIVED - Novel Hybridization System For Canola (MS8/RF3)

MS8/RF3 hybrid canola differs from non-transgenic canola only in the insertion of three new genes, barnase, barstar and bar, and in the ...

#33. Notification C/BE/96/01 Oilseed Rape Ms8xRf3

(Barnase protein produced by the barnase gene), and a DNA sequence limiting its expression to a specific stage and time during the development of the ...

#34. A general approach for the generation of orthogonal tRNAs

Background: The addition of new amino acids to the genetic ... amber nonsense mutations in the barnase gene. The remaining. tRNATyr.

#35. Executive Summary (pdf 617 kb) - Food Standards

napus producing areas of Canada, USA and Australia. The bar, barnase and barstar genes were introduced into the B. napus genome in a single gene construct via ...

#36. PSI Plasmid of the Month - DNASU |

In order to propagate vectors containing the barnase gene, Escherichia coli strains containing the detoxifying barstar gene must be used (e.g. BR610). Currently ...

#37. Study on Pollen Fertility in Transgenic Maize with ... - JIPB

PCR assay showed that 18 plants were positive for Barnase gene with a frequency of 75%. I-KI staining and pollen germination revealed that five plants were ...

#38. Restoring pollen fertility in transgenic male-sterile eggplant by

Four T0-plants with the Barnase gene were obtained, all proved to be male-sterile and incapable of pro- ducing viable pollen. Flowers stamens were shorter, ...

#39. Best 1 Definitions of Barnase - YourDictionary

The transgenic construct included a barnase ribonuclease gene controlled by a tapetum promoter. Bejo Zaden BV Male sterile radicchio rosso The barnase gene from ...

#40. TA29-Barnase基因导致抗虫转基因欧洲黑杨雄性不育的研究

A STUDY ON THE INTRODUCTION OF MALE STERILITY OF ANTIINSECT TRANSGENIC Populus nigra BY TA29-BARNASE GENE. Li Ling,Qi Liwang,Han Yifan,Wang Yingchun,Li ...

#41. Hybrid Cultivar Development - 第 187 頁 - Google 圖書結果

For developing these chimeric genes , the anther - specific promotor TA29 was fused separately with RNase - Tl from Aspergillus oryzae and the barnase gene ...

#42. The Science of Horticulture 01 - 第 116 頁 - Google 圖書結果

The Barnase gene of Bacillus amyloliquefaciens encodes an RNAase . When Barnase gene is driven by TA29 promoter , it is expressed only in tapetum cells ...

#43. barnase - German translation - Linguee

External sources (not reviewed). the barnase gene from Bacillus [...].

#44. Advances in DNA Research and Application: 2012 Edition

“A series of betasatellite/split mutant barnase gene constructs, in which a direct repeat of the Bacillus amyloliquefaciens barnase gene flanked the ...

#45. Nature of the tapetum-specific promoter is crucial for ... - PubAg

... for generation of rice transgenics possessing a lethal barnase gene ... OsG6b promoter had successfully generated 100% male sterile barnase line, ...

#46. Crystal structural analysis of a barnase-barstar complex at 2.0 ...

Electrostatic interaction investigations of the Barnase-Barstar complex ... Molecular Functions of Genetic Variants Linked with Diseases.

#47. Cotton Genetic Transformation of Barnase Male Sterility Gene

棉 花 学 报 Cotton Science 2007，19(4)：261-266. Cotton Genetic Transformation of Barnase Male Sterility Gene ZHANG Hui-jun 1 ，WANG Huan-yu 2 ，SHI ...

#48. Barnase barstar system - SlideShare

Induced GMS Barnase gene is cytotoxic killing the tapetul cells, thus preventing pollen development result transgenic male sterility. 27. Each ...

#49. Engineering the AEG1 promoter from cotton to develop male ...

barnase and barstar genes for developing male sterile and restorer ... roots making it unusable to drive the expression of barnase gene.

#50. 31996D0424 - EN - EUR-Lex

(i) the barnase gene from Bacillus amyloliquefaciens (a ribonuclease) with ... of the nopaline synthase gene from Agrobacterium tumefaciens;.

#51. Barnase-Barstar Pair: Contemporary Application in Cancer ...

The identification of barnase intracellular inhibitor barstar led to ... Using barnase in suicide gene therapy has also found its niche in ...

#52. Bioluminescence Resonance Energy Transfer (BRET) Allows ...

The genes encoding the fusion proteins barnase–luciferase (RLuc) and barstar–. EGFP (an enhanced variant of Aequoria victoria green fluorescent ...

#53. Us & Them, the farmers | The Indian Express

One reason is because the alien Barnase and Barstar genes are both isolated from a soil bacterium, making for a mustard that is transgenic ...

#54. Assessment of genetically modified oilseed rape MS11 for ...

The pat/bar gene originates from S. hygroscopicus, and the barnase and barstar genes originate from B. amyloliquefaciens, none of which are considered ...

#55. [SANET-MG] barnase is toxic to humans and other mammals

The bacterial gene for barnase and the gene for barstar have been employed extensively in transgenic plants and animals to set up regulatory systems that ...

#56. A new phagemid vector for positive selection of recombinants ...

KS+ phagemid in which the modified barnase killer gene has been fused downstream from the lac promoter of the pBluescript. II KS+ multiple restriction site.

#57. Bacillus velezensis UCMB-5033 barnase inhibitor - BioCyc

Derived by automated computational analysis using gene prediction method: Protein Homology. Molecular Weight of Polypeptide, 11.475 kD (from nucleotide sequence) ...

#58. Development and characterization of transgenic ... - J-Stage

In this study, we generated transgenic dominant male sterile rice using the barnase gene expressed by the tapetum-. Breeding Science. Preview. Page 3 ...

#59. Technology : Suicidal potatoes make sacrifice to save crop

promoter turns on the barnase gene and kills it. However, the researchers found that even a healthy, genetically modified plant makes small ...

#60. Examples of 'barnase' in a sentence - Collins Dictionary

The barnase gene and barnase-barstar operon were successfully amplified in one. Reindert Nijland, Michael J. Hall, J. Grant Burgess.

#61. 초록 - 한국학술지인용색인

Nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene - Hybrid seed production;Male sterile ...

#62. Active barnase variants with completely random hydrophobic ...

synbar, a synthetic barnase gene designed to produce mature barnase in the cytoplasm ofE. coli (Fig. 2). Amber stop codons,.

#63. Frequently Asked Questions (FAQs)

Centre for Genetic Manipulation of Crop Plants (CGMCP), University of Delhi, South Campus ... Both Barnase and Barstar encoding genes were expressed under a.

#64. Supplementary Methods

barnase and barstar fusion proteins are based on the vector pIG6-4D5 (refs 1-3). The barstar and barnase genes were amplified out of the plasmid pMT413 (ref ...

#65. Retransformation of a male sterile barnase line with the ...

Double transformants carrying both the barnase and barstar genes were identified and screened for their male fertility status. Using this ...

#66. pF25A ICE T7 Flexi® Vector Protocol - Promega Corporation

The lethal barnase gene for positive selection of the insert. ... in E. coli once the barnase gene has been replaced with the protein-coding sequence of ...

#67. The Killing Fields: Terminator Crops at Large

A search on the US database on field trials [2] revealed that similar male sterile lines engineered with the 'terminator-gene', barnase (see below), ...

#68. A genetic selection for compensating mutations at the barnase ...

controlled to keep the mutant barnase gene silent. When expression of barnase is turned on, failure to form a complex between the mutant barnase and barstar ...

#69. pFC7A (HQ) Sequence and Map - SnapGene

... BtgI BtgI PvuI PvuI PvuI AsiSI AsiSI AsiSI 70 barnase barnase barnase G C G A T C G C C A C G C T A G C G G T ... Fluorescent Protein Genes & Plasmids.

#70. Genetically engineered male sterile crop plants may ... - Toppr

Chimaeric ribonuclease gene (barnase gene) expression within the anther selectively destroys the tapetal cell layer that surrounds the pollen sac, ...

#71. barstar gene | testbiotech

The genetic modification encompasses three genes derived from bacterial and fungal sources: ... Genes: barnase gene · barstar gene · bar gene ...

#72. Decisions taken in the 73rd Meeting of the Genetic ... - GEAC

objectives of (a) development of male sterile lines in B juncea by fusing barnase gene and (b) restoration of male sterility by barstar ...

#73. Creating Completely Both Male and Female Sterile Plants by ...

We then developed an efficient method to specifically ablate microspore and megaspore mother cells using the SDS and BARNASE fusion gene, which resulted in ...

#74. Barnase - Barstar System of Male Sterility - YouTube

Barnase - Barstar System of Male Sterility | Vikas Mangal ... Barnase -Barstar Transgenic Genetic Male Sterility/Pollination Control.

#75. Production of Engineered Pelargonium plants

... Engineered long-lived and male sterile plants; - ipt gene: expressed in long-living plants; - Barnase: gene expressed in pollen free plants ...

#76. Development of barnase/barstar transgenics for hybrid seed ...

transgenics with barnase and barstar genes respectively24. ... the combiners by expressing the barnase gene from Ba-.

#77. Most recent papers with the keyword Barnase - Read by QxMD

A promoter expressing in anther and roots is made anther specific. The modified promoter is used to drive barnase gene and develop male sterile lines.

#78. TA29-barnase基因转化菜心 - 期刊界

The pTA29-barnase Chimeric Gene Transformation of Brassica campestris L. subsp. chinensis Makino var. parachinensis Mediated by Agrobacterium.

#79. Detection method details - EUGINIUS

Name: QL-ELE-B'nase-F-n4/BNaseR-n3. Description: Qualitative real-time PCR (TaqMan) method for detection of the barnase gene (Scholtens et al. 2013).

#80. Genetics and Plant Breeding - Facebook

The system utilizes the expression of barnase gene, encoding cytotoxic RNase protein in the tapetum cells to make one of the combiner's male sterile.

#81. Reference - RRC(Research Resource Circulation)

Barnase is the extracellular ribonuclease of Bacillus amyloliquefaciens and barstar its specific intracellular inhibitor. The gene for barstar has now been ...

#82. Barnase - Wikiwand

Barnase is a bacterial protein that consists of 110 amino acids and has ribonuclease activity. It is synthesized and secreted by the bacterium Bacillus ...